Virus Induced Gene Silencing of Candidate Genes in N. benthamiana

(Hailing Jin, UC Berkeley)
A total of 55 candidate resistance and resistance pathway genes were subjected to functional characterization using virus-induced gene silencing (VIGS). Forty-five candidate genes were chosen from literature searches that may play a role in plant disease resistance and plant-microbial interaction. Six genes were chosen from the list of differentially expressed genes from microarray analysis on NPK1- and Sgt1-silenced N. benthamiana. Four genes were selected from the sequence information of potato BAC clones. Solanaceae N. benthamiana was used for VIGS analysis. Candidate genes were selected from data reported using Arabidopsis, tomato, potato, tobacco, yeast and animals.